Organogenesis from shoot segments and via callus of endangered <Emphasis Type="Italic">Kosteletzkya pentacarpos</Emphasis> (L.) Ledeb. |
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Authors: | Anna Piovan Rosy Caniato Elsa Mariella Cappelletti Raffaella Filippini |
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Institution: | (1) Department of Biology, University of Padua, via U. Bassi 58/B, 35121 Padua, Italy;(2) Botanical Garden, University of Padua, via Orto Botanico 15, 35123 Padua, Italy; |
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Abstract: | Efficient plant regeneration systems both from shoot segments and via callus organogenesis were developed for Kosteletzkya pentacarpos (L.) Ledeb., a rare and endangered Eurasian species. In the experiments with existing meristems, factors affecting shoot
proliferation, including explant type, i.e. decapitated and intact shoots, and plant growth regulators, indole-3-acetic acid
or kinetin, were investigated. Shoot proliferation was significantly affected by the type of explant, the hormones and their
interaction. The highest shoot multiplication rate was obtained from decapitated shoots. Increasing kinetin concentration
promoted shoot elongation regardless of explant type. In intact shoots, shoot length was also affected by increasing auxin
concentration, although this effect tends to decrease with higher concentration. Decapitated shoots were not responsive to
the addition of auxin. Micropropagation through organogenesis from callus was also investigated. Calli were obtained from
leaf, stem internode and root explants. Only the leaf-derived calli produced shoots and indole-3-acetic acid favoured increased
numbers of shoots. A number of experiments were conducted for rooting of in vitro produced shoots. All of them induced high
rooting frequency, the number and the length of roots being dependent on the strength of the basal medium. The use of 1–2 mg l−1 indole-3-butyric acid resulted in refining the optimal concentration for root elongation. The regenerated plants (70%) survived
and flowered in their first vegetative period. |
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