Two novel disaccharides, rutinose and methylrutinose, are involved in carbon metabolism in Datisca glomerata |
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Authors: | Maria Schubert Anna N Melnikova Nikola Mesecke Elena K Zubkova Rocco Fortte Denis R Batashev Inga Barth Norbert Sauer Yuri V Gamalei Natalia S Mamushina Lutz F Tietze Olga V Voitsekhovskaja Katharina Pawlowski |
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Institution: | 1. Albrecht von Haller Institute for Plant Sciences, Plant Biochemistry, G?ttingen University, 37077, G?ttingen, Germany 2. Komarov Botanical Institute, Russian Academy of Sciences, 197376, St. Petersburg, Russia 3. Institute of Organic and Biomolecular Chemistry, G?ttingen University, 37077, G?ttingen, Germany 4. Department of Molecular Plant Physiology, University of Erlangen-Nürnberg, 90158, Erlangen, Germany 5. Department of Botany, Stockholm University, 10691, Stockholm, Sweden
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Abstract: | Datisca glomerata forms nitrogen-fixing root nodules in symbiosis with soil actinomycetes from the genus Frankia. Analysis of sugars in roots, nodules and leaves of D. glomerata revealed the presence of two novel compounds that were identified as α-l-rhamnopyranoside-(1 → 6)-d-glucose (rutinose) and α-l-rhamnopyranoside-(1 → 6)-1-O-β-d-methylglucose (methylrutinose). Rutinose has been found previously as a/the glycoside part of several flavonoid glycosides, e.g. rutin, also of datiscin, the main flavonoid of Datisca cannabina, but had not been reported as free sugar. Time course analyses suggest that both rutinose and methylrutinose might play a role in transient carbon storage in sink organs and, to a lesser extent, in source leaves. Their concentrations show that they can accumulate in the vacuole. Rutinose, but not methylrutinose, was accepted as a substrate by the tonoplast disaccharide transporter SUT4 from Arabidopsis. In vivo 14C-labeling and the study of uptake of exogenous sucrose and rutinose from the leaf apoplast showed that neither rutinose nor methylrutinose appreciably participate in phloem translocation of carbon from source to sink organs, despite rutinose being found in the apoplast at significant levels. A model for sugar metabolism in D. glomerata is presented. |
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