Two loci required for cytoplasmic organization in early embryos of Caenorhabditis elegans |
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| Authors: | K J Kemphues N Wolf W B Wood D Hirsh |
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| Institution: | 1. Departments of Molecular Pharmacology and Neurosciences, Albert Einstein College of Medicine, NY, USA;2. The Neuro- Lab, School of Health and Health Technology, Federal University of Technology, Akure, Nigeria;1. Department of Medicine, Section of Cardiac Electrophysiology, Emory University School of Medicine, Atlanta, Georgia;2. Department of Medicine, St. George''s University School of Medicine, Grenada, West Indies;3. Emory University, Rollins School of Biostatistics and Bioinformatics, Atlanta, Georgia;4. Department of Cardiology, Essentia Health, Duluth, Minnesota;1. Department of Energy and Biotechnology, Myongji University, Yongin 449-728, Republic of Korea;2. Department of Food and Nutrition, Myongji University, Yongin 449-728, Republic of Korea;1. College of Biotechnology, Dalian Polytechnic University, Dalian, People''s Republic of China;2. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun, People''s Republic of China;3. Institute for Brain Disorders, Dalian Medical University, Dalian, People''s Republic of China;1. Biomarker Branch, Research Institute, National Cancer Center, Gyeonggi, Republic of Korea;2. Department of Cancer Biomedical Science, Graduate School of Cancer Science and Policy, National Cancer Center, Gyeonggi, Republic of Korea;3. Department of Pharmaceutical and Biomedical Engineering, Cheongju University, Cheongju, Chungcheongbuk-do 28503, Republic of Korea |
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| Abstract: | We have identified five new alleles, including an amber allele, at each of two loci (zyg-11 II and zyg-9 II) previously identified by temperature-sensitive strict maternal-effect lethal mutations. Genetic analysis indicates that each of these genes is expressed specifically during oogenesis and encodes a protein product whose function is required only during embryogenesis. Temperature-pulse experiments suggest that the time of action of both products is during the one-cell stage of embryogenesis. Phenotypic analysis reveals that mutations in both loci lead to disorganization of the cytoplasm in early embryos and to abnormalities in at least one of the meiotic divisions. Mutations at the zyg-9 locus appear to specifically affect microtubule function in one-cell embryos while zyg-11 mutations affect many cytoplasmic properties. |
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