蝗虫染色体分带技术的研究

本文对采自长沙岳麓山的中华稻蝗〔Oxya chinensis (Thunberg)〕和采自杭州的小稻蝗〔Oxya hyla intricata (Stal) 〕进行了染色体C带、R带、N带和A带的处理研究。结果表明，C带显示结构异染色质，其位置在不同分裂相中较为稳定；R带即G带的反带，带纹较C带丰富，在粗线期最为明显，本文观察到不同的温育条件可影响R带产生的效果；N带和Ag带可相互参照比较，从而为NOR的准确定位提供依据；A带即荧光带，本文对其显带程序作了一定的改进。Abstract:In this paperOxya chinensis (Thunberg) collected from the Yuelu Mountains in Changsha, Hunan Province andOxya hyla intricata (Stal) collected from Hangzhou, Zhejiang province were analysed by chromosomal C-banding、R-banding、N-banding、Ag-banding and A-banding. The results showed that the constitutive heterochromatin was stained in C-banding, and it relatively stable; R-banding was the reverse of G-banding, its bands were relatively obvious at the pachytene and abundent, it may be because of the selective extracts that cause the bands evident, the different effects were surveyed under the various incubation conditions; Both N-banding and Ag-banding can stain the nucleolar organizer and the two were compared in this paper; The A-banding was a little improved in the light of the banding process.

A Study of Locust's Chromosomal Banding Technology