Global changes in genomic methylation levels during early development of the zebrafish embryo |
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| Authors: | A?A?Mhanni Email author" target="_blank">R?A?McGowanEmail author |
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| Institution: | (1) Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2;(2) Department of Zoology, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2;(3) Present address: Division of Medical Genetics, Royal University Hospital, Room 515, Ellis Hall, 103 Hospital Drive, Saskatoon, SK, Canada, S7N 0W8;(4) Departments of Biochemistry and Biology, Memorial University of Newfoundland, St. John s, NL, Canada, A1C 5S7 |
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| Abstract: | We have examined the methylation status of the zebrafish genome during early embryogenesis and we find evidence that methylation fluxes do occur in that organism. The parental genetic contributions to the zygote are, initially, differently methylated with the genome of the sperm being hypermethylated relative to the genome of the oocyte. Post-fertilization there is an immediate decrease in methylation of the embryonic genome but the methylation begins to increase rapidly and is re-established by the gastrulation stage. These results are consistent with the results of Santos et al. (Dev Biol 241:172–182, 2002), who examined the methylation of early mouse embryos, and this conservation argues that demethylation/re-methylation is an important part of vertebrate development.Edited by D. Tautz |
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| Keywords: | Zebrafish Methylation Embryogenesis Epigenetics |
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