Evidence for the presence of a reversible Ca2+-dependent pore activated by oxidative stress in heart mitochondria. |
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Authors: | M Crompton A Costi L Hayat |
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Affiliation: | Department of Biochemistry, University College London, U.K. |
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Abstract: | Rat heart mitochondria became permeabilized to sucrose when incubated with 100 nmol of Ca2+/mg of protein in the presence of Pi. Ca2+ chelation with EGTA restored impermeability to sucrose, which became entrapped in the matrix space. t-Butylhydroperoxide markedly promoted permeabilization in the presence of Ca2+ but not in its absence, and Ca2+-plus-t-butylhydroperoxide-induced permeabilization was reversed by EGTA. The data suggest that Ca2+ and oxidative stress synergistically promote the reversible opening of an inner membrane pore. |
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