Reconstruction and expression of the MRI-contrast protein,ferritin, with recombinant rabies vectors |
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| Authors: | Lingling Liu Kai Gong Pinggang Ming Ying Huang Qing Tang Gelin Xu Jiaxin Yan Nanming Zhao Xiufang Zhang Yandao Gong |
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| Institution: | (1) State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing, 100084, China;(2) State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, China CDC, 100 Ying Xin St, Xuan Wu District, Beijing, 100052, China;(3) Department of Genetic Engineering, Wuhan Institute of Biological Product, 9 Linjiang St, Wu Chang District, 443006 Wuhan, China |
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| Abstract: | Attenuated recombinant rabies vector could be an ideal system for delivery of contrast agent gene for Magnetic Resonance Imaging
(MRI) because of its neurotropic nature. In this study, the gene of a biomolecular contrast agent, ferritin, was successfully
cloned into two rabies virus vectors, vaccine-based pCTN and street strain-based pNH. Recombinant virus granules were obtained
and proved to express ferritin by RT-PCR after transfection of CTN-ferritin and NH-ferritin vector systems in BHK-21 cells.
The recovered rabies virus-rCTN-ferritin was of similar ability to rNH-ferritin, which suggests the possibility of application
of this safe and effective rabies vector system in delivery of diagnostic or therapeutic genes into the brain. |
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