Proteomic alterations of <Emphasis Type="Italic">Brassica napus</Emphasis> root in response to boron deficiency |
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Authors: | Zhifang Wang Zhenhua Wang Lei Shi Lijun Wang Fangsen Xu |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China;(2) Microelement Research Centre, Huazhong Agricultural University, 430070 Wuhan, China; |
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Abstract: | Boron (B) deficiency is a worldwide problem, and Brassica napus is one of the most sensitive crops to B deficiency. To better understand the B starvation response of Brassica napus, we conducted a comparative proteomic analysis of seedling stage Brassica napus root between B-sufficient and B-limited conditions: 45 differentially expressed proteins were successfully identified by
2-DE coupled with MALDI-TOF/TOF-MS and LTQ-ESI-MS/MS analysis. Among these proteins, 10 were down-regulated and 35 were up-regulated
under B-limited condition. Combining GO and KEGG analyses with data from previous reports, proteins were categorized into
several functional groups, including antioxidant and detoxification, defense-related proteins, signaling and regulation, carbohydrate
and energy metabolism, amino acid and fatty acid metabolism, protein translation and degradation, cell wall structure, and
transporter. The genes of selected proteins were analyzed by quantitative RT-PCR. Our results provide novel information for
better understanding the physiological and biochemical responses to B deficiency in plants. |
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Keywords: | |
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