Disruption of Ca2+ Homeostasis In Trypanosoma Cruzi By Crystal Violet |
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Authors: | ROBERTO DOCAMPOA FERNANDA R GADELHA SILVIA N J MORENO GUSTAVO BENAIM MARIA E HOFFMANN ANIBAL E VERCESI |
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Institution: | Department of Veterinary Pathobiology, University of Illinois, 2001 S. Lincoln Avenue, Urbana, Illinois 61801 USA;Centro de Biología Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela, and;Departmento de Bioquímica, Universidade Estadual de Campinas, Campinas, SP 13081, Brazil |
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Abstract: | ABSTRACT. We have demonstrated previously that crystal violet induces a rapid, dose-related collapse of the inner mitochondrial membrane potential of Trypanosoma cruzi epimastigotes. In this work, we show that crystal violet-induced dissipation of the membrane potential was accompanied by an efflux of Ca2+ from the mitochondria. In addition, crystal violet inhibited the ATP-dependent, oligomycin-, and antimycin A-insensitive Ca2+ uptake by digitonin-permeabilired epimastigotes. Crystal violet also induced Ca2+ release from the mitochondria and endoplasmic reticulum of digitonin-permeabilized trypomastigotes. Furthermore, crystal violet inhibited Ca2+ uptake and the (Ca2+-Mg2+)ATPase of a highly enriched plasma membrane fraction of epimastigotes, thus indicating an inhibition of other calcium transport mechanisms of the cells. Disruption of Ca2+ homeostasis by crystal violet may be a key process leading to trypanosome cell injury by this drug. |
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Keywords: | Supplementary key words Mitochondria plasma membrane Ca2+-ATPase fura-2 |
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