Simultaneous determination of d- and l-propranolol in human plasma by high-performance liquid chromatography |
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Authors: | Jörgen Hermansson Christer Von Bahr |
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Affiliation: | National Board of Health and Welfare, Department of Drugs, Division of Pharmacy, Box 607, S-751 25 Uppsala Sweden;Department of Clinical Pharmacology, Karolinska Institute, Huddinge Hospital, S-141 86 Huddinge Sweden |
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Abstract: | A method for the determination of d- and l-propranolol in human plasma is described. The method involves extraction of propranolol from plasma, and the formation of diastereomeric derivatives with the chiral reagent N-trifluoroacetyl-1-prolylchloride. Separation and quantitation of the diastereomeric propranolol derivatives are carried out by a reversed-phase high-performance liquid-chromatographic system with fluorimetric detection. The reproducibility in the determination of d- and l-propranolol in human plasma was 4.5% (relative standard deviation) at drug levels of 10 ng/ml.In two subjects who received a single 40-mg tablet of racemic propranolol the plasma levels of the d-isomer were lower than of the l-propranolol. The half-lives of d- and l-propranolol were similar. |
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