Sensitive gas chromatographic assay for the quantitation of bretylium in plasma,urine and myocardial tissue |
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| Authors: | Eugene Patterson Philip Stetson Benedict P. Lucchesi |
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| Affiliation: | Department of Pharmacology and The Upjohn Center for Clinical Pharmacology, The University of Michigan Medical School, Ann Arbor, Mich. 48109 U.S.A. |
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| Abstract: | A sensitive analytical method has been developed for the quantitation of bretylium in plasma, urine and myocardial tissue. Bretylium and the internal standard, UM-360 (o-iodobenzyltrimethylammonium), are extracted and isolated as the iodide salts. Sodium benzenethiolate is added and the mixture heated to 100° for one hour. This results in the formation of 2-bromobenzyl phenyl thioether and 2-iodobenzyl phenyl thioether, which can be separated and quantitated by gas chromatography. Good reliability and reproducibility can be obtained using electron-capture detection with quantities of bretylium as small as 1 ng. |
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| Keywords: | To whom correspondence should be addressed |
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