Mass fragmentographic determination of xanthine and hypoxanthine in biological fluids |
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Authors: | J.L. Chabard C. Lartigue-Mattei F. Vedrine J. Petit J.A. Berger |
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Affiliation: | Laboratoire de Chimie analytique de la Faculté de Pharmacie et Unité No 71 de l''I.N.S.E.R.M., Boite Postale No 38, 63001 Clermont-Ferrand Cedex France |
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Abstract: | The method presented for the simultaneous determination of xanthine and hypoxanthine, uses mass-fragmentography in the electron impact (EI) mode, after the gas chromatographic separation of butylated derivatives. Butylation, rather than methylation, is used in order to avoid interference coming from exogenous caffeine, which is frequently encountered. [7,9-15N]Xanthine is used as the internal standard, and for each sample, a blank is obtained by xanthine oxidase reaction. In the biological fluids studied the sensitivity was about 50 ng/ml. |
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