Improving the catalytic performance of fungal laccases in monoterpene-based reaction systems |
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Authors: | Aikaterini A Tzialla Ali A Taha Emmanuel Kalogeris and Haralambos Stamatis |
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Institution: | (1) Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece;(2) Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 15780 Athens, Greece |
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Abstract: | Ternary systems consisting of monoterpenes (α-pinene or d-limonene), tert-butanol and water were used as reaction media to enhance the catalytic performance of laccases from various fungi sources
(Trametes versicolor, T. hirsuta and Botrytis cinerea). The enzymes had improved catalytic efficiency (5- to 10-fold) in α-pinene-rich environment, while optimal reaction rates were in high-water content systems (15.5% v/v). The stability of laccases
was significantly improved in monoterpene-based systems (up to 90% residual enzyme activity after 24 h at 30°C) in comparison
with other non-conventional media. The results indicate that these ternary systems can increase the potential of laccases
as catalysts for various oxidations. |
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Keywords: | Laccases Stabilization Ternary systems Terpenes |
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