Optimization of solid-phase synthesis of difficult peptide sequences via comparison between different improved approaches |
| |
Authors: | S Abdel Rahman A El-Kafrawy A Hattaba M F Anwer |
| |
Institution: | (1) Faculty of Science, Zagazig University, Zagazig, Egypt |
| |
Abstract: | Summary. Different approaches are applied to avoid the strong aggregation of the difficult peptide sequences, which is considered as
the main reason for incomplete acylation and deprotection reactions hindering the synthesis of these sequences.
Temporary protection of amide nitrogen of peptide bond using 2-hydroxy-4-methoxybenzyl (Hmb) and 2,4,6-timethoxybenzyl (Tmob)
amino acid derivatives, introduction of D-Ala or Pro residues in the peptide chain sequences and utilization of microwave
energy are proved to be useful methods in the enhancement of solubility and in the hindrance of the aggregation during the
solid-phase synthesis of oligoalanine. Oligoalanine is chosen to demonstrate the difficult sequences and to compare the efficiencies
of these methods. |
| |
Keywords: | : Difficult peptide sequences – Solid-phase peptide synthesis – Oligo-alanine – N-amide protection – Microwave energy |
本文献已被 PubMed SpringerLink 等数据库收录! |