A study of the methods available for the cytochemical localization of histamine by fluorescence induced with o-phthalaldehyde or acetaldehyde |
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| Authors: | S. A. M. Cross S. W. B. Ewen F. W. D. Rost |
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| Affiliation: | (1) Research Institute, Smith Kline & French Laboratories Limited, Welwyn Garden City, Herts.;(2) Department of Histochemistry, Royal Postgraduate Medical School, London;(3) Present address: Department of Pathology, University of Aberdeen, UK |
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| Abstract: | Synopsis Methods for the histochemical localization of histamine were investigated, tissues and models being exposed to vapours ofo-phthalaldehyde, acetaldehyde, or glutaraldehyde and afterwards examined by fluorescence microscopy. Acetaldehyde and glutaraldehyde induce fluorescence in histamine models, but the method is not sufficiently sensitive for use in tissues. A modification of theo-phthalaldehyde method, giving improved reliability, is described in detail and some results in rat and dog stomach are presented. |
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