| 格尔德霉素生物合成的调控基因 |
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| 引用本文： | 赫卫清,雷健,刘玉瑛,王以光. 格尔德霉素生物合成的调控基因[J]. 微生物学报, 2008, 24(5): 717-722 |
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| 作者姓名： | 赫卫清 雷健 刘玉瑛 王以光 |
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| 作者单位： | 中国医学科学院/中国协和医科大学医药生物技术研究所, 卫生部抗生素生物工程重点实验室, 北京 100050;中国医学科学院/中国协和医科大学医药生物技术研究所, 卫生部抗生素生物工程重点实验室, 北京 100050;中国医学科学院/中国协和医科大学医药生物技术研究所, 卫生部抗生素生物工程重点实验室, 北京 100050;中国医学科学院/中国协和医科大学医药生物技术研究所, 卫生部抗生素生物工程重点实验室, 北京 100050 |
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| 基金项目： | 基础研究重大项目前期研究专项(973项目)(No. 2001CCA00500)。 |
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| 摘 要： | 从吸水链霉菌17997中克隆了格尔德霉素(Geldanamycin, Gdm)生物合成酶基因簇, 通过生物信息学分析发现两个LAL(Large ATP-binding regulators of the LuxR family)家族的调控基因gdmRI和gdmRII, 基因阻断和基因回复实验证实这两个基因产物都正调控Gdm的生物合成。
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| 关 键 词： | 格尔德霉素(Geldanamycin Gdm) 调控基因 基因阻断 |
Regulatory Genes of Geldanamycin Biosynthesis |
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| Weiqing He,Jian Lei,Yuying Liu and Yiguang Wang. Regulatory Genes of Geldanamycin Biosynthesis[J]. Acta microbiologica Sinica, 2008, 24(5): 717-722 |
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| Authors: | Weiqing He Jian Lei Yuying Liu Yiguang Wang |
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| Affiliation: | Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China |
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| Abstract: | Two LAL family regulatory genes, gdmRI and gdmRII, were identified in the geldanamycin biosynthetic gene cluster of Streptomyces hygroscopicus 17997. Disruption of the two regulatory genes resulted in absolute elimination of geldanamycin biosynthesis. The complementation experiments using a single wild-type gene could restore geldanamycin production. These results indicated that both gdmRI and gdmRII were positive regulatory genes of the geldanamycin biosynthesis. |
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| Keywords: | geldanamycin (Gdm) regulatory gene gene disruption |
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