The problem of determining the affinity of bivalent antibodies using ELISA and its mathematical solution] |
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| Authors: | S A Bobrovnik |
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| Affiliation: | A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine. |
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| Abstract: | A new approach for the determination of the affinity of monovalent receptors in direct coordinates could be obtained without using Klotz or Scatchard equations. The mathematical solution of the problem of the determination of the affinity of bivalent antibodies by ELISA gives the following relation between absorbance, measured in ELISA. and antigen concentration: omega + square root of omega 2 + omega = liK, where omega = (Ao-Ai)/Ai, Ai is the absorbance, if Ag concentration is equal li; Ao is the absorbance, if Ag concentration is equal zero, K is an equilibrium constant. |
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