Aurora B kinase cooperates with CENP-E to promote timely anaphase onset |
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Authors: | André F Maia Tália Feijão Martijn J M Vromans Claudio E Sunkel Susanne M A Lens |
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Institution: | 1. IBMC—Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180, Porto, Portugal 2. Department of Medical Oncology, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands 3. ICBAS—Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003, Porto, Portugal
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Abstract: | Error-free chromosome segregation requires that all chromosomes biorient on the mitotic spindle. The motor protein Centromere-associated
protein E (CENP-E) facilitates chromosome congression by mediating the lateral sliding of sister chromatids along existing
K-fibers, while the mitotic kinase Aurora B detaches kinetochore–microtubule interactions that are not bioriented. Whether
these activities cooperate to promote efficient chromosome biorientation and timely anaphase onset is not known. We here show
that the chromosomes that fail to congress after CENP-E depletion displayed high centromeric Aurora B kinase activity. This
activity destabilized spindle pole proximal kinetochore–microtubule interactions resulting in a checkpoint-dependent mitotic
delay that allowed CENP-E-independent chromosome congression, thus reducing chromosome segregation errors. This shows that
Aurora B keeps the mitotic checkpoint active by destabilizing kinetochore fibers of polar chromosomes to permit chromosome
congression in CENP-E-compromised cells and implies that this kinase normally prevents pole proximal syntelic attachments
to allow CENP-E-mediated congression of mono-oriented chromosomes. |
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