婴幼儿腹泻A群轮状病毒G和P的基因分型研究

目的研究浙江萧山医院婴幼儿童腹泻标本中人轮状病毒（Human Rotavims）毒株的感染情况及G和P基因型流行特点。方法收集该院2009年8月至2010年8月腹泻儿童15233份粪便标本采用酶联免疫吸附试验、逆转录-巢式聚合酶链反应进行轮状病毒病原检测，将128份阳性标本进行VP7和VP4基本分型。结果15233份婴幼儿腹泻标本中有2706份标本为轮状病毒阳性，阳性率17．8％；男孩和女孩检出率差异无统计学意义，以6-12月龄段检出率最高；对128份阳性标本进行G血清分型和P基因分型，G1型53份（41．4％）、G3型38份（29．7％）、G1G3型17份（13．3％）、G未分型20份（15．6％）；P[8]型72份（56．3％）、P[4]型16份（12．5％）、P[8]P[4]型3份（2．3％）、P未分型37份（28．9％），G血清型和P基因型的组合以G1P[8]为主，占29．7％（38／128）。结论浙江萧山医院A群轮状病毒G血清以G1型为主，其次为G3型，P基因型以P[8]型为主。

Molecular genotyping analysis of group A rotavirus in children

Objective To analyze the epidemic characteristics of group A rotavirus in children by serotyping G and genotyping P.Method 15 233 samples of patients with diarrhea under 5years from August 2009 to August 2010 were collected for detection of rotavirus.The 128 rotavirus antigen-positive stool specimens were enrolled for serotyping G and genotyping P by RT-Nest PCR.Result The rotavirus existed in 17.7%(2706/15233) of the stool specimens.There was no significant differences between genders.The highest detectable rate exsited in children aged 6-12 month.Of the total 128 G serotyping,G1 accounted for 41.4%,G3 29.7%,G1G3 13.3% and non-typeable 15.6%.72(56.3%) of the strains were P[8],followed by 16(12.5%) P[4],and a total of 38(29.7%) isolates were found to be non-typeable.G1P[8] was the predominant combination,with the proportion of 29.7%.Conclusion G1 and P[8] are the most common serotype and genotype of group A rotavirus in our hospital.