细菌性阴道病的优势菌群半定量培养分析

目的探讨临床适用的菌群培养方法,用于检测分析细菌性阴道病患者阴道优势菌群,以更好地指导临床诊治。方法采集32例细菌性阴道病（BV）患者的阴道分泌物标本,在不同气体环境中用非选择性、半定量方法做细菌培养,比较培养结果,分析患者阴道优势菌群。结果在厌氧环境中培养时菌落数量最多,检出的优势菌共15种,每份标本的优势细菌种类多数为2种（20/32）,少数为3种（3/32）;而在微需氧及需氧环境中检出的主要菌分别为8种及5种。结论非选择性半定量、厌氧培养的方法可有效、简便地了解BV阴道优势菌群的构成,可用于临床对BV菌群的检测分析研究。

Semi-quantitative culture analysis of predominating flora of bacterial vaginosis

Objective To evaluate the cultivation methods for vaginal flora in clinical practice with the aim to detect and analyze vaginal predominating flora from women with bacterial vaginosis(BV),so as to better guide clinical diagnosis and treatment.Method The vaginal fluid samples were taken from 32 BV patients,and cultivated on non-selective media using the semi-quantitative method under different air conditions.The results were compared so as to analyze patients′ vaginal predominating flora.Result The largest number of bacteria derived from anaerobic conditions,and there were a total of 15 predominating bacteria species characterized,with mainly 2(20/32) or sometimes 3(3/32) species per specimen.While there were 8 and 5 dominant bacteria species characterized in microaerobic and aerobic conditions,respectively.Conclusion The composition of BV vaginal predominating flora can be determined using non-selective,semi-quantitative and anaerobic culture techniques effectively and simply,so the method can be clinically used for detection,analysis and study of BV-related bacteria flora