| 云南兰坪铅锌矿区细菌多样性研究 |
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| 引用本文： | 邓伟,季秀玲,袁文功,李波,林连兵,张琦,魏云林. 云南兰坪铅锌矿区细菌多样性研究[J]. 中国微生态学杂志, 2012, 24(2): 109-112 |
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| 作者姓名： | 邓伟 季秀玲 袁文功 李波 林连兵 张琦 魏云林 |
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| 作者单位： | 1. 昆明理工大学生物工程技术研究中心,云南昆明,650504
2. 昆明理工大学国土资源工程学院,云南昆明,650504 |
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| 基金项目： | 云南省自然科学基金(KKSA200826046);云南省后备人才基金(2009CI027) |
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| 摘 要： | 目的研究云南省兰坪铅锌矿区矿石样品中的细菌群落结构。方法构建细菌16S rRNA高变区基因文库。结果矿石内部细菌种类丰富多样,文库测序结果表明矿石样品中细菌分属于4个门,27个属;其中变形菌门（Proteobacteria）类群是优势种群,占所有细菌总克隆数的51.7%,而且大多数属于γ-变形菌纲亚群。各克隆序列分析结果表明,其中部分细菌序列与已报道物种的相似性较低。结论表明云南省兰坪铅锌矿矿石样品中可能存在新的细菌种类。
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| 关 键 词： | 兰坪铅锌矿 16S rRNA基因文库 细菌多样性 |
Diversity of bacteria in Lanping Zinc-lead ore in Yunnan province |
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| DENG Wei , JI Xiu-ling , YUAN Wen-gong , LI Bo , LIN Lian-bing , ZHANG Qi , WEI Yun-lin. Diversity of bacteria in Lanping Zinc-lead ore in Yunnan province[J]. Chinese Journal of Microecology, 2012, 24(2): 109-112 |
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| Authors: | DENG Wei JI Xiu-ling YUAN Wen-gong LI Bo LIN Lian-bing ZHANG Qi WEI Yun-lin |
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| Affiliation: | 1(1.Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650093,China;2.Faculty of Land Resource Engineering,Kunming University of Science and Technology,Kunming 650093,China) |
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| Abstract: | Objective To study the bacterial community structure and diversity in ore samples from Lanping Zinc-lead Mine in Yunnan province.Method 16S rRNA gene library was constructed.Result A diverse bacterial community inhabits in the ore samples was revealed.Sequence analysis results showed that the library contained 4 bacterial phyla including 27 genera.Phylogenetic tree was constructed to confirm these results.The most abundant phylum was Proteobacteria,which accounted for 51.7% of all the obtained clones,and the most abundant subgroup within Proteobacteria was γ-Proteobacteria.NCBI BLAST analysis showed that some clones exhibited low levels of DNA similarity with any of the known 16S rDNA sequences in the database.Conclusion Some new species may exit in the ore. |
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| Keywords: | Lanping zine-lead ore 16S rRNA gene library Bacterial diversity |
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