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Deuterium-exchange metabolomics identifies N-methyl lyso phosphatidylethanolamines as abundant lipids in acidophilic mixed microbial communities |
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| Authors: | Curt R. Fischer Paul Wilmes Benjamin P. Bowen Trent R. Northen Jillian F. Banfield |
| Affiliation: | 1. Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA, 94720, USA 4. Ginkgo Bioworks, Boston, MA, USA 5. Department of Environment and Agro-biotechnologies, Public Research Centre??Gabriel Lippmann, and Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Belvaux, Grand-Duchy of Luxembourg 3. Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA 2. Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA |
| Abstract: | Natural microbial communities are extremely diverse and contain uncharacterized but functionally important small molecules. By coupling a deuterium (D) labeling technique to high mass accuracy untargeted liquid chromatography-electrospray ionization-mass spectrometry (LC?CESI?CMS) metabolomic analysis, we found that natural acidophilic microbial biofilms dominated by bacteria of the genus Leptospirillum contained unusual lyso phosphatidylethanolamine (PE) lipids in high abundance (more than 10?nmol/mg of dry biomass). The unusual polar head group structure of these lipids is similar to lipids found in phylogenetically unrelated acidophilic chemoautolithotrophs and may be related to the affinity of these lipids for iron and calcium ions. Correlations of lyso phospholipid and proteome abundance patterns suggest a link between the lyso phospholipids and the UBA-type substrain of Leptospirillum group II. By combining untargeted metabolomics with D exchange we demonstrate the ability to identify cryptic but biologically functional small molecules in mixed microbial communities. |
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