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The binding mechanisms of plasma protein to active compounds in Alismaorientale rhizomes (Alismatis Rhizoma)
Institution:1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;2. Center for Analysis and Testing, Nanjing Normal University, Nanjing 210097, China;3. College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, China;1. Department of Central Scientific Services, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India;2. Department of Zoology, Baruipur College, South 24 parganas 743 610, India;3. Department of Physical Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India;4. Department of Metallurgy & Materials Engineering, Bengal Engineering and Science University, Shibpur, Howrah 711 103, India;1. IRIT – Université de Toulouse, Université Paul Sabatier, 118 Route de Narbonne, F-31062 Toulouse Cedex 9, France;2. FAYOL-EMSE, CNRS-UMR5600, EVS, Ecole Nationale Supérieure des Mines, F-42023 Saint-Etienne, France;1. Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan;2. Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Nagatsuta-cho 4259 G1-13, Midori-ku, Yokohama 226-8502, Japan;3. FIRST (Faculty of Frontiers of Innovative Research in Science and Technology), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan;1. Geophysical Laboratory, Carnegie Institution of Washington, 5251 Broad Branch Road NW, Washington, DC 20015-9801, USA;2. Department of Earth and Planetary Science, University of California, Berkeley, 479 McCone Hall, Berkeley, CA 94720-4767, USA;3. Berkeley Geochronology Center, 2455 Ridge Road, Berkeley, CA 94709, USA;4. Department of Earth, Environment, and Planetary Sciences, Brown University, 324 Brook Street, Providence, RI 02912, USA;5. Department of Earth Sciences, University of Oxford, South Parks Road, Oxford OX1 3AN, UK
Abstract:Ultrafiltration and HPLC were employed to assess binding rates between rat plasma protein and two active compounds with lipid-regulating properties (alisol B 23-acetate and alisol A 24-acetate) from Alismaorientale rhizomes (Alismatis Rhizoma), a traditional Chinese medicine. SDS–PAGE was used for the evaluation of the binding between the alisol acetates and Hb in plasma. The fluorescence spectroscopy and circular dichroism spectroscopy were also combined with molecular modeling to explore binding mechanisms between Hb and the alisol acetates under imitative physiological condition. The ultrafiltration results show that alisol B 23-acetate bound more strongly than alisol A 24-acetate to plasma protein. SDS–PAGE results may suggest that alisols bind to Hb in plasma. The spectroscopy results are consisting with the molecular modeling results, and they indicate that the differences in plasma protein binding strength between the two compounds may be related to their side chains. A folded side chain/parent ring bound more strongly to Hb than an open side chain/parent ring.
Keywords:Active compounds in Alismatis Rhizoma  Ultrafiltration  SDS–PAGE  Fluorescence spectroscopy  Circular dichroism spectroscopy  Molecular modeling
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