Modulation of binding properties of amphiphilic DNA containing multiple dodecyl phosphotriester linkages to lipid bilayer membrane |
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| Affiliation: | 1. Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, United States;2. Program in Craniofacial Biology, University of California, San Francisco, San Francisco, CA, United States;3. Department of Pediatrics, University of California, San Francisco, San Francisco, CA, United States;4. Endocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, San Francisco, CA, United States;5. Institute for Human Genetics, University of California, San Francisco, San Francisco, CA, United States |
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| Abstract: | DNA is a promising functional molecule to modify and design lipid membrane functions. In order to use DNA in a hydrophilic–hydrophobic interface including lipid membrane, we have developed an amphiphilic DNA having dodecyl phosphotriester linkages (dod-DNA). Herein, we report the binding of a series of amphiphilic dod-DNAs to the lipid bilayer membrane. Surface plasmon resonance (SPR) assay and fluorescent microscopy showed that dod-DNA having three dodecyl groups at each end strongly bound to lipid membrane due to the slow dissociation rate and the dod-DNA can be used as a linear template for molecular arrangement on the membrane surface. |
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| Keywords: | Amphiphilic DNA Lipid membrane Liposome SPR |
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