Facile synthesis,cytotoxicity and bioimaging of Fe3+ selective fluorescent chemosensor |
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Affiliation: | 1. Department of Chemistry, Kongju National University, Gongju, Chungnam 314-701, Republic of Korea;2. Department of Plasma Bioscience and Display, Kwangwoon University, 20 Kwangwoon-gil, Nowon-gu, Seoul 139-701, Republic of Korea;1. Catholic University of Louvain, Georges Lemaître Centre for Earth and Climate Research, Belgium;2. Research Institute of Geodesy, Topography and Cartography, Geodetic Observatory Pecný, Czech Republic;3. Department of Meteorology and Geophysics, University of Vienna, Austria;4. State Key Laboratory of Geodesy and Earth''s Dynamics, Institute of Geodesy and Geophysics, CAS, Wuhan, China;1. Laboratory of Hydraulics, Hydrology and Glaciology, Swiss Federal Institute of Technology Zürich, Switzerland;2. Department of Agricultural Engineering, Federal University of Ceará, Brazil;3. Institute for Building Materials, Swiss Federal Institute of Technology Zürich, Switzerland |
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Abstract: | The designing and development of fluorescent chemosensors have recently been intensively explored for sensitive and specific detection of environmentally and biologically relevant metal ions in aqueous solution and living cells. Herein, we report the photophysical results of alanine substituted rhodamine B derivative 3 having specific binding affinity toward Fe3+ with micro molar concentration level. Through fluorescence titration at 599 nm, we were confirmed that ligand 3 exhibited ratiometric fluorescence response with remarkable enhancement in emission intensity by complexation between 3 and Fe3+ while it appeared no emission in case of the competitive ions (Sc3+, Yb3+, In3+, Ce3+, Sm3+, Cr3+, Sn2+, Pb2+, Ni2+, Co2+, Cu2+, Ba2+, Ca2+, Mg2+, Ag+, Cs+, Cu+, K+) in aqueous/methanol (60:40, v/v) at neutral pH. However, the fluorescence as well as colorimetric response of ligand–iron complex solution was quenched by addition of KCN which snatches the Fe3+ from complex and turn off the sensor confirming the recognition process was reversible. Furthermore, bioimaging studies against L-929 cells (mouse fibroblast cells) and BHK-21 (hamster kidney fibroblast), through confocal fluorescence microscopic experiment indicated that ligand showed good permeability and minimum toxicity against the tested cell lines. |
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Keywords: | Turn on fluorescent chemosensor Bioimaging Cell permeability Toxicity assay KCN induced fluorescence quenching |
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