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A novel aggregation-induced emission fluorescent probe for nucleic acid detection and its applications in cell imaging
Institution:1. Center of Bio & Micro/Nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, PR China;2. School of Information Science and Engineering, Shandong University, Jinan 250100, PR China;3. Department of Anatomy, Shandong University School of Medicine, Jinan 250012, PR China;1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei, 430072, PR China;2. College of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, PR China;3. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, PR China;1. Key Laboratory of Biomedical Polymers-Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;2. The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China;1. State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116023, China;2. Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China;1. Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Road, BaoShan District, Shanghai, 200444, China;2. Department of Materials Science and NanoEngineering, Rice University, 6100 Main Street, Houston, TX, 77005, USA;3. International Centre for Quantum and Molecular Structures, Physics Department, Shanghai University, 99 Shangda Road, BaoShan District, Shanghai, 200444, China;4. School of Life Science and Technology, Tongji University, 1239 Siping Road, Yangpu District, Shanghai, 200082, China;5. Beijing National Laboratory for Condensed Matter Physics and Institute of Physics, Chinese Academy of Sciences, No.8 3rd South Street, Zhongguancun Haidian District, Beijing, 100190, China;6. Key Laboratory of MEMS of Ministry of Education, School of Electronic Science and Engineering, Southeast University, 2 Sipailou Road, Xuanwu District, Nanjing, 210096, China;7. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science, Guangxi Normal University, 15 Yucai Road, Qixing District, Guilin, 541004, China
Abstract:A new kind of aggregation-induced emission compound was synthesized and used as the probe of nucleic acid. The characterization of this compound was studied. Both the RNA and DNA were detected by using this probe. And the detection scope of DNA and RNA was different. We researched the selectivity of our probe in double and single strand DNA sequences. The visualization of gel electrophoresis and the cell nucleus imaging were researched as well. Compared with the traditional nucleus dye Hoechst 33258, our probe also has the potential to be nucleus dye. And the cell toxicity was well performed by MTT assays.
Keywords:Fluorescent  Nucleic acid  Aggregation-induced emission  Gel electrophoresis  Cell imaging  Dye
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