Artificial enzymes with protein scaffolds: Structural design and modification |
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| Institution: | 1. Department of Mechanical Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA;2. Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA;3. Department of Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA;4. Department of Nanoengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA |
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| Abstract: | Recent development in biochemical experiment techniques and bioinformatics has enabled us to create a variety of artificial biocatalysts with protein scaffolds (namely ‘artificial enzymes’). The construction methods of these catalysts include genetic mutation, chemical modification using synthetic molecules and/or a combination of these methods. Designed evolution strategy based on the structural information of host proteins has become more and more popular as an effective approach to construct artificial protein-based biocatalysts with desired reactivities. From the viewpoint of application of artificial enzymes for organic synthesis, recently constructed artificial enzymes mediating oxidation, reduction and C–C bond formation/cleavage are introduced in this review article. |
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| Keywords: | Biocatalyst Artificial enzyme Oxidation Reduction C–C bond formation/cleavage |
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