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Preparation of ferrocene-functionalized gold nanoparticles by primer extension reaction on the particle surface |
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| Institution: | 1. Department of Gynecologic Oncology, Saitama Medical University International Medical Center, 1397-1 Yamane, Hidaka, Saitama 350-1298, Japan;2. Department of Pathology, Saitama Medical University International Medical Center, 1397-1 Yamane, Hidaka, Saitama 350-1298, Japan;1. College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China;2. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China |
| Abstract: | DNA molecules possessing multiple ferrocene (Fc) molecules as a redox active probe were prepared by the primer extension (PEX) reaction using a 2′-deoxyuridine-5′-triphosphate derivative in which Fc was connected to the C5-position of the uridine by a diethylene glycol linker. Gold nanoparticles (AuNP) covered with DNA possessing the Fc molecules were prepared by the PEX reaction on the surface. The AuNP–FcDNA conjugates exhibit a detectable electrochemical signal due to the Fc molecules. Possible application of the PEX reaction on AuNP is demonstrated for the detection of a single nucleotide mutation in the target DNA. |
| Keywords: | Polymerase extension reaction DNA Ferrocene Gold nanoparticles Electrochemistry |
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