Involvement of OmpF during reception and translocation steps of colicin N entry |
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Authors: | J-P Bourdineaud H-P Frerobe C Lazdunski J-M Pagès |
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Institution: | Centre de Biochimie et de Biologie Moléculaire, CNRS, Marseille, France. |
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Abstract: | 125I]-colicin N binds to OmpF receptor sites (70,000 per cell) with an average Kassoc of 3.2 x 10(6) M-1 at 23 degrees C. Monoclonal antibody directed against a cell-surface-exposed epitope of OmpF is able to complete with the binding of the colicin in vitro and also to protect against colicin N in vivo. OmpF is an absolute requirement for colicin N uptake. OmpC cannot serve as a substitute for OmpF during translocation across the outer membrane under receptor bypass conditions, which is in contrast to colicin A. Colicin N does not cross-react with various monoclonal antibodies directed against colicin A. |
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