Optimization of ultrasonic-assisted extraction process of Poria cocos polysaccharides by response surface methodology |
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Authors: | Wang Yongjiang Cheng Zhong Mao Jianwei Fan Minger Wu Xueqian |
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Institution: | aKey Laboratory of Agricultural Products Chemical and Biological Processing Technology of Zhejiang Province, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, PR China;bZhejiang Province Academy of Forestry, Hangzhou 310023, PR China |
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Abstract: | Polysaccharides production from Poria cocos was carried out using aqueous NaOH with the assistance of ultrasonic. Experimental design was used to investigate the effect of three parameters (extraction time, extraction concentration of NaOH, and ratio of aqueous NaOH to raw material) on polysaccharides yields. The ranges of the factors investigated were 1–3 min for extraction time (X1), 0.5–1.0 mol/L for extraction concentration of NaOH (X2), and 30–50 for ratio of aqueous NaOH to raw material (X3). The statistical analysis of the experiment indicated that extraction concentration of NaOH had significant effect on P. cocos polysaccharides yields. The central composite design showed that polynomial regression models were in good agreement with the experimental results with the coefficients of determination of 0.9935 for P. cocos polysaccharides yield. The optimal condition for P. cocos polysaccharides yield within the experimental range of the variables studied was at 2.44 min, 0.789 mol/L, and 53.0. At this condition, the predicted yield of polysaccharides extracted was 82.3%. |
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Keywords: | Poria cocos polysaccharides Extraction Central composite design Optimization Response surface methodology Correlation |
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