Physical mapping of the pea chloroplast DNA and localization of the ribosomal RNA genes |
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| Authors: | N M Chu K K Oishi K K Tewari |
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| Institution: | Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California 92717 USA |
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| Abstract: | The closed circular DNA of pea chloroplast has been digested with restriction endonucleases SalI, SmaI, BamHI, XbaI, XhoI, HindIII, and EcoRI. A physical restriction map of pea ctDNA has been constructed by mapping the SalI and SmaI sites. The pea ctDNA has been found to contain one set of ribosomal RNA genes by Southern hybridization of restriction endonuclease digest, R-loop studies, and DNA-DNA heteroduplex mapping. The 23 S and 16 S RNA genes are confined to a DNA region of 3.0 and 1.5 kbp, respectively. The two rRNA chains are separated by a spacer region of 2.2 kbp. |
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