In situ fluorescence monitoring of immobilizedClostridium acetobutylicum |
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| Authors: | Kenneth F Reardon Thomas Scheper James E Bailey |
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| Institution: | (1) Department of Chemical Engineering, California Institute of Technology, 91125 Pasadena, California, USA;(2) Present address: Institut für Technische Chemie, Universität Hannover, D-3000 Hannover 1, Federal Republic of Germany |
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| Abstract: | The NAD(P)H-dependent culture fluorescence of immobilizedClostridium acetobutylicum was followed during a three-part fermentation involving product formation on minimal and nitrogen-free media. The fluorescence signal, together with a knowledge of the metabolic pathways ofCl. acetobutylicum, provices information on the state and intracellular activities of the immobilized bacteria not readily found by other methods. |
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