Identification of Sodium Chloride-Regulated Genes in <Emphasis Type="Italic">Burkholderia cenocepacia</Emphasis> |
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Authors: | Shantanu Bhatt Christine L Weingart |
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Institution: | (1) Department of Microbiology and Molecular Genetics, Emory University, Atlanta, GA 30322, USA;(2) Department of Biology, Samson Talbot Hall of Biological Science, Denison University, Granville, OH 43023, USA |
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Abstract: | Previous studies have suggested that the airways of cystic fibrosis (CF) patients have elevated sodium chloride (NaCl) levels
due to the malfunctioning of the CF transmembrane conductance regulator protein. For bacteria to survive in this high-salt
environment, they must adjust by altering the regulation of gene expression. Among the different bacteria inhabiting the airways
of CF patients is the opportunistic pathogen Burkholderia cenocepacia. Previous studies have indicated that B. cenocepacia produces a toxin and cable pili under high osmolar conditions. We used transposon mutagenesis to identify NaCl-regulated
genes in the clinical strain B. cenocepacia K56-2. Six transconjugants were induced with increasing NaCl concentration. The DNA flanking the transposon was sequenced
and five distinct open reading frames were identified encoding the following putative proteins: an integrase, an NAD-dependent
deacetylase, TolB, an oxidoreductase, and a novel hypothetical protein. The collective results of this study provide important
information about the physiology of B. cenocepacia when faced with osmotic stress and suggest the identity of significant virulence mechanisms in this opportunistic pathogen. |
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