Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen |
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Authors: | Boyle Sarah Kolin David L Bieler Joan Glick Schneck Jonathan P Wiseman Paul W Edidin Michael |
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Institution: | †Biology Department, The Johns Hopkins University, Baltimore, Maryland;‡Department of Physics, McGill University, Montreal, Canada;§Pathology Department, Johns Hopkins Medical Institutions, Baltimore, Maryland |
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Abstract: | Changes in the clustering of surface receptors modulate cell responses to ligands. Hence, global measures of receptor clustering can be useful for characterizing cell states. Using T cell receptor for antigen as an example, we show that k-space image correlation spectroscopy of quantum dots blinking detects T cell receptor clusters on a scale of tens of nanometers and reports changes in clustering after T cell activation. Our results offer a general approach to the global analysis of lateral organization and receptor clustering in single cells, and can thus be applied when the cell type of interest is rare. |
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