Self-synchronization of the protein synthesis rhythm in HaCaT cultures of human keratinocytes |
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Authors: | V Ya Brodskii V V Terskikh A V Vasilyev N D Zvezdina E A Vorotelyak V I Fateeva L A Mal’chenko |
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Institution: | 1.Kol’tsov Institute of Developmental Biology,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | In cultures of human keratinocytes HaCaT contained in a serum-free medium on glass, a circahoralian rhythm of protein synthesis
was found similar to the one in hepatocytes in vitro. The intensity of the synthesis was determined by the inclusion of 3H-leucine corrected for the pool of free marked leucine. Rhythm was studied in washed 1- or 2-day cultures after the change
of the medium. The medium conditioned with keratinocytes HaCaT synchronized the rarefied hepatocyte cultures nonsynchronous
in the control. Therefore, the keratinocytes liberate synchronizing factors into the medium. A BAPTA-AM chelator of calcium
ions eliminates the protein synthesis rhythm both in dense hepatocyte cultures synchronous in the control and in the HaCaT
keratinocyte cultures. The effect of the H7 inhibitor of protein kinases was analogous. Thus, both in keratinocytes and hepatocytes,
self-synchronization of fluctuations of the intensity of protein synthesis takes place. The mechanism of self-synchronization
is the calcium-depending phosphorylation of cell proteins. |
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