Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats |
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Authors: | Marta Fernández-Galilea Patricia Pérez-Matute Pedro Prieto-Hontoria J Alfredo Martínez María Jesús Moreno-Aliaga |
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Institution: | 1.Department of Nutrition, Food Science, Physiology and Toxicology,University of Navarra,Pamplona,Spain;2.HIV and Associated Metabolic Alterations Unit. Infectious Diseases Area,Center for Biomedical Research of La Rioja (CIBIR),Logro?o,Spain |
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Abstract: | Lipoic acid (LA) is an antioxidant with therapeutic properties on several diseases like diabetes and obesity. Apelin is a
novel adipokine with potential beneficial actions on glucose metabolism and insulin resistance. The aim of this study was
to examine in 3T3-L1 adipocytes the effects of LA on apelin gene expression and secretion, as well as elucidate the signaling
pathways involved. We also tested the regulation of adipose apelin gene expression by LA supplementation in a model of high-fat
diet-induced obesity. LA increased apelin secretion but not apelin gene expression in 3T3-L1 adipocytes. The AMPK inhibitor
Compound C induced an increase in LA-stimulated apelin production, and, on the contrary, the AMPK activator AICAR completely
reversed the LA stimulatory effects on apelin secretion, also inducing a significant reduction in apelin mRNA levels in this
in vitro model. Apelin mRNA levels were increased in those animals fed with the high-fat diet, while the caloric restriction
decreased apelin mRNA to control levels. However, apelin gene expression was not significantly modified in rats treated with
LA compared with the obese group. The current data suggest the ability of LA to modulate apelin secretion by adipocytes. However
the insulin-sensitizing effect of LA in vivo is not related to changes in apelin gene expression in our model of diet-induced
obesity. |
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