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Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale |
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| Authors: | Valdés Rodolfo Reyes Biunayki Alvarez Tatiana García Janet Montero José A Figueroa Alejandro Gómez Leonardo Padilla Sigifredo Geada Déborah Abrahantes Ma Carmen Dorta Lamay Fernández Damián Mendoza Otto Ramirez Nadia Rodriguez Marylin Pujol Merardo Borroto Carlos Brito José |
| Affiliation: | Monoclonal Antibody Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba. |
| Abstract: | This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice. |
| Keywords: | Plant-derived antibody Monoclonal antibody Transgenic plant Hepatitis B virus surface antigen Immunoaffinity |
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