Battery of tests for profiling abnormalities of vitamin K-dependent coagulation factors in drug-toxicity studies in rats. |
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| Authors: | Masaaki Kurata Takeshi Iidaka Naoko Yamasaki Yukiko Sasayama Yoshimasa Hamada |
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| Affiliation: | Worldwide Safety Sciences, Pfizer Global Research and Development, Nagoya Laboratories, Pfizer Inc, Aichi, Japan. |
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| Abstract: | A battery of simple tests for profiling abnormalities of vitamin K-dependent coagulation factors encountered in drug-toxicity studies was verified in rats treated with warfarin (3 and 10 mg/kg, p.o). The thrombotest, or hepaplastin-test, is useful as a follow-up test after routine screening tests for coagulation abnormalities based on PT and APTT, to rule out other coagulation-factor abnormalities. Measurement of coagulation factor activities (factors II, VII, IX and X) using factor-deficient human plasmas provides direct evidence of decreased activities of vitamin K-dependent factors. Furthermore, Echis carinatus venom coagulation time, together with factor II activity, allows us to confirm the generation of PIVKA-II. |
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