| 厚荚相思树根瘤菌HJ06菌株的16S rDNA全序列和nifA基因片段分析 |
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| 引用本文： | 吕成群,黄宝灵,庄培亮,武 波.厚荚相思树根瘤菌HJ06菌株的16S rDNA全序列和nifA基因片段分析[J].热带亚热带植物学报,2006,14(1):19-24. |
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| 作者姓名： | 吕成群 黄宝灵 庄培亮 武 波 |
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| 作者单位： | 1. 广西大学林学院,南宁,530005
2. 广西大学生命科学与技术学院,南宁,530005 |
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| 基金项目： | 广西自然科学基金;广西林业科研项目 |
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| 摘 要： | 对厚荚相思(Acaciacrassicarpa)根瘤菌HJ06菌株的16SrDNA全序列和nifA基因片段进行了测定。结果表明,HJ06菌株在以16SrDNA序列构建的系统发育树状图中位于根瘤菌属(Rhizobium)分支中,与根瘤菌属各个种的相似性达95%以上;从HJ06菌株克隆出的585bpnifA基因片段与Klebsiellapneumoniae的同源性达到99.3%,与Klebsiellaoxytoca的NifF,NifL,NifA,NifB蛋白基因的同源性为97.8%。
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| 关 键 词： | 厚荚相思 根瘤菌 16SrDNA全序列 系统发育 nifA基因 |
| 文章编号： | 1005-3395（2006）01-0019-06 |
| 收稿时间： | 2005-06-14 |
| 修稿时间： | 2005-12-01 |
16S rDNA and nifA Gene Sequence Analysis of Rhizobium Strain HJ06 Isolated from Acacia crassicarpa |
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| Lü Cheng-qun,HUANG Bao-ling,ZHUANG Pei-liang,WU Bo.16S rDNA and nifA Gene Sequence Analysis of Rhizobium Strain HJ06 Isolated from Acacia crassicarpa[J].Journal of Tropical and Subtropical Botany,2006,14(1):19-24. |
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| Authors: | Lü Cheng-qun HUANG Bao-ling ZHUANG Pei-liang WU Bo |
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| Institution: | a. Forestry College, Guangxi University; b. College of Life Science and Teehnology, Guangxi University, Nanning 530005, China |
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| Abstract: | The determination of full-length 16S rDNA sequence and nifA gene segments of root nodule bacterium strain HJ06 isolated from Acacia crassicarpa showed that strain HJ06 had above 95% similarity with the genus Rhizobium. The nifA gene segments of HJ06 have 99.3% isogenesis with nifA genes of Klebsiella pneumoniae and 97.8% isogenesis with genes of NifF, NifL, NifA and NifB proteins of K. oxytoca. |
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| Keywords: | Acacia crassicarpa Rhizobium 16S rDNA sequencing Phylogenetic analysis nifA gene$$ |
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