Energy depletion and autophagy. Cytochemical and biochemical studies in isolated rat hepatocytes |
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Authors: | Jacques P. M. Schellens and Alfred J. Meijer |
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Affiliation: | (1) Laboratory of Cell Biology and Histology and E. C. Slater Institute for Biochemical Research, Academic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands |
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Abstract: | Summary In this paper, data dealing with the sensitivity of autophagy towards partial ATP depletion in isolated rat hepatocytes are reviewed. Partial reduction of intracellular ATP causes: (1) a decrease of proteolytic flux; (2) a decrease in uptake of cytosolic components into the autophagic-lysosomal compartment; (3) either a decrease or no change in the ratio between volume densities of autophagosomes and lysosomes, depending on whether or not the cytosolic phosphate potential is affected; and (4) impairment of the lysosomal proton pump. It is concluded that the consecutive steps of autophagy all respond to relatively small changes of intracellular ATP concentration. |
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