| 中国首次分离到Kadipiro病毒 |
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| 引用本文： | 孙肖红,孟维珊,付士红,冯云,翟友刚,王静林,王焕琴,吕新军,梁国栋.中国首次分离到Kadipiro病毒[J].病毒学报,2009,25(3):173-177. |
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| 作者姓名： | 孙肖红 孟维珊 付士红 冯云 翟友刚 王静林 王焕琴 吕新军 梁国栋 |
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| 作者单位： | 中国疾病预防控制中心,病毒病预防控制所,北京,100052;云南省地方病防治所,云南,大理,671000 |
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| 基金项目： | 国家科技部重大传染病专项(2003BA712A08-01);;中法先进研究计划(B06-04);;中美传染病合作项目(U19-GH000004) |
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| 摘 要： | 在云南省西北部地区进行的虫媒病毒调查中,从三带喙库蚊、中华按蚊和骚扰阿蚊中分别分离到5株病毒。全部病毒在C6/36细胞上产生细胞病变,而在BHK21细胞不产生细胞病变;电子显微镜观察可见病毒颗粒呈球形,直径约70nm(n=7),无包膜、表面存在明显刺状突起;聚丙烯酰胺凝胶电泳显示分离的病毒为12节段双链RNA病毒,病毒基因组带形呈6-5-1分布;用Kadipiro病毒的特异性引物可以扩增到目的条带,基因组第12节段全长758nt,与Kadipiro病毒原型株JKT-7075的同源性为90%,遗传进化分析显示在云南蚊虫分离的5株病毒均为呼肠孤病毒科(Reoviridae)东南亚十二节段RNA病毒属(Seadornavirus)Kadipiro病毒。本文为我国Kadipiro病毒分离的首次报道。
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| 关 键 词： | Kadipiro病毒 分离 鉴定 |
The First Report of Kadipiro Virus Isolation in China |
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| SUN Xiao-hong,MENG Wei-shan,FU Shi-hong,FENG Yun,ZHAI You-gang,WANG Jing-lin,WANG Huan-qin,LV Xin-jun,LIANG Guo-dong.The First Report of Kadipiro Virus Isolation in China[J].Chinese Journal of Virology,2009,25(3):173-177. |
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| Authors: | SUN Xiao-hong MENG Wei-shan FU Shi-hong FENG Yun ZHAI You-gang WANG Jing-lin WANG Huan-qin LV Xin-jun LIANG Guo-dong |
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| Institution: | 1.Institute for Viral Disease Control and Prevention;Chinese Center for Disease Control and Prevention;Beijing 100052;China;2.Yunnan Provincial Institute of Endemic Diseases Control and Prevention;Dali 671000;China |
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| Abstract: | 5 strains of virus isolated from Culex tritaeniorhynchus, Anopheles sinensis and Armigeres subalbatus, which caused cytopathic effect in C6/36 cells, had been obtained in the survey of arboviruses in Northwestern Yunnan Province. China. The virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. RNA-PAGE of the genomes of the isolates showed 6-5-1 profile. A fragment of the 12th segment sequence was amplified by a pair of specific primers for Kadipiro virus strain... |
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| Keywords: | Kadipiro virus Isolation Identification |
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