Simple detection method for distinguishing dead and living yeast colonies |
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| Authors: | Kucsera J Yarita K Takeo K |
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| Affiliation: | Department of Ultrastructure and Molecular Function, Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan. kucsera@bio.u-szeged.hu |
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| Abstract: | A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies. |
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