| 利用PCR方法从人染色体DNA中扩增G-CSF基因 |
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| 引用本文： | 卢一凡,邓继先,肖成祖,马清钧. 利用PCR方法从人染色体DNA中扩增G-CSF基因[J]. 生物技术通讯, 1996, 0(4) |
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| 作者姓名： | 卢一凡 邓继先 肖成祖 马清钧 |
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| 作者单位： | 军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所 北京 100071，北京 100071，北京 100071，北京 100071 |
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| 摘 要： | 利用PCR技术从染色体基因组DNA中扩增大DNA片段具有相当大的难度。本试验采用碱变性模板以及热启动等方法,成功地扩增出1.5kb的人基因组DNA,并讨论了影响扩增大DNA片段特异性和产量的因素。
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| 关 键 词： | PCR扩增 G-CSF 特异性 |
Studies on the PCR Amplification of G-CSF Gene from Human Genomic DNA |
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| Lu Yifan,Deng Jixian,Xiao Chengzhu. Studies on the PCR Amplification of G-CSF Gene from Human Genomic DNA[J]. Letters in Biotechnology, 1996, 0(4) |
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| Authors: | Lu Yifan Deng Jixian Xiao Chengzhu |
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| Abstract: | It is usually quite difficult to amplify long DNA fragment from genomic DNA. In this paper, Some improved methods, such as denaturating template by NaOH and hotting start, were successfully used to amplify the 1. 5kb G-CSF gene from human genomic DNA , and some important factors affecting the amplification of long DNA fragment were discussed. |
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| Keywords: | PCR amplification G-CSF Specificity |
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