Effects of Low Temperature, Light and O2 on Chilling-sensitive and -resistant Strains in Chlorella ellipsoidea |
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| Authors: | Sadakane, Haruo Ishibashi, Kazuko Yoshimoto, Makoto Hatano, Shoji |
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| Affiliation: | 1 Department of Food Science and Technology, Faculty of Agriculture, Kyushu University Fukuoka 812, Japan
2 Faculty of Pharmacology, Fukuoka University Fukuoka 814, Japan |
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| Abstract: | A low-temperature sensitive strain, Chlorella ellipsoidea Gerneck(IAM C-102), lost its chilling sensitivity during preservation.Cells of the original strain (low-temperature sensitive) andthe variant (low-temperature resistant) were both synchronouslygrown under a 14-hr light-10-hr dark regime. In the originalstrain, cells at the D-L stage (transient phase) were most sensitiveto a low temperature, whereas the variant cells were not damagedat any stage. During low-temperature treatment, the viability of D-L cellsin the sensitive strain decreased after a lag period of 1 hr.The O2-uptake activity (respiration) showed the same behavioras the viability, whereas the O2-evolution activity (photosynthesis)decreased from the start of chilling. In the resistant strain,only O2 evolution decreased. The decreased activity was restoredwhen the chilled cells were incubated at 25°C. This restorationwas inhibited by oligomycin. Lowering the light intensity or eliminating O2 diminished thechilling injury of the sensitive strain. The results indicatethat the chilling injury of Chlorella results from the combinedeffects of low temperature, light and O2. (Received September 26, 1980; Accepted March 23, 1981) |
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