A general method for isolation of individual polyribosomes and pure mRNAs by immunosorption technique |
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| Authors: | E V Sidorova M G Trudolyubova M I Lerman |
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| Institution: | (1) N. F. Gamaleya Institute of Epidemiology and Microbiology, Institute of Biological and Medical Chemistry, Academy of Medical Sciences of the U.S.S.R., Moscow, U.S.S.R. |
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| Abstract: | A highly specific procedure has been developed for the isolation of individual polyribosomes by immunosorbents containing immobilized antigen-antibody complexes. Polyribosomes synthesizing immunoglobulin IgGl and serum albumin were quantificated and isolated in the native state from MOPC 21A plasmacytoma and rat liver cells. For albumin polyribosomes, the presumptive messenger RNA was demonstrated; it contained three components: 20S, 16S and 9S.Abbreviations IgG
murine immunoglobulin
- RSA
rat serum albumin
- RGG
rat gamma-globulin
- EDTA
ethylene diamine tetraacetate
- SDS
sodium dodecyl sulphate |
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