Excitation contraction coupling in skeletal muscle: evidence for a role of slow Ca2+ channels using Ca2+ channel activators and inhibitors in the dihydropyridine series |
| |
| Authors: | M Ildefonse V Jacquemond O Rougier J F Renaud M Fosset M Lazdunski |
| |
| Affiliation: | 1. Université Claude Bernard, 43 bd du 11 novembre 1918 69621 Villeurbanne Cedex, France;2. Université de Nice, Centre de Biochimie du CNRS, Parc Valrose 06034 Nice Cedex, France;1. Department of Urology, Zhongnan Hospital of Wuhan University, TaiKang Center for Life and Medical Sciences, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China;2. Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA;3. Beijing Frontier Research Center for Biological Structures, Tsinghua-Peking Center for Life Sciences, State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China;4. Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Institute of Biology, Westlake Institute for Advanced Study, Zhejiang Provincial Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang 310024, China;5. Center for Quantitative Biology, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China;6. Tsinghua-Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China;7. Department of Biological Sciences, St. John’s University, Queens, NY 11439, USA;8. Shenzhen Medical Academy of Research and Translation, Shenzhen, Guangdong 518107, China;1. Key Laboratory of Clean Pulp & Papermaking and Pollution Control of Guangxi, College of Light Industry and Food Engineering Guangxi, Guangxi University, Nanning, 530004, PR China;2. Department of Chemical Engineering, College of Environmental Science and Forestry, State University of New York, Syracuse, NY, 13210, United States;1. LUNAM Université, Ecole des Mines de Nantes, IRCCyN UMR CNRS 6597 (Institut de Recherche en Communications et Cybernétique de Nantes), Nantes, France, grid.449623.e;2. EIGSI, 26 Rue François de Vaux de Foletier, La Rochelle, France, 0000 0001 0018 053X, grid.462149.c;1. Department of Bioengineering, Imperial College London, SW7 2AZ London, UK;2. Faculty of Electrical Engineering and Computer Science, University of Maribor, Koroška cesta 46, 2000 Maribor, Slovenia;3. Centre of Precision Rehabilitation for Spinal Pain (CPR Spine), School of Sport, Exercise and Rehabilitation Sciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, UK;4. Department of Integrative Physiology, University of Colorado Boulder, CO, United States;5. Department of Movement, Human and Health Sciences, University of Rome “Foro Italico”, Rome, Italy;1. Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China;2. HKUST Shenzhen-Hong Kong Collaborative Innovation Research Institute, Shenzhen, Guangdong, China;1. State Key Laboratory of Advanced Metallurgy, University of Science and Technology Beijing, Beijing 100083, China;2. School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083, China |
| |
| Abstract: | Ca2+ current and tension have been simultaneously recorded from single twitch fibres of the semi-tendinosus of Rana esculenta in a medium containing a physiological Ca2+ concentration (1.8 mM). Under appropriate conditions it can be shown that tension develops in two phases. The first is rapid and reaches its maximum before activation of the inward Ca2+ current. The second phase is slower and with a time course which appears to be correlated with that of the inward current. Nifedipine, a specific Ca2+ channel inhibitor greatly reduced ICa2+ and the slower component of tension. Bay K8644 a Ca2+ channel activator, which has receptors on T-tubule, increased ICa2+ and the slow component of tension. These results indicate that a slow component of skeletal muscle contraction is related to the inward Ca2+ current flowing through dihydropyridine sensitive voltage-dependent Ca2+ channels. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
