|
|||||
|
|
Functional assay for shiga-like toxin via detection by antibody capture and multivalent galabiose binding |
|
| Authors: | Hilde van Hattum Kim van der Zwaluw Gerben M. Visser Jos van Putten Rob Ruijtenbeek Roland J. Pieters |
| Affiliation: | 1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands;2. Centre for Infectious Disease Control, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands;3. Department of Infectious Diseases and Immunology, Utrecht University, 3584 CL Utrecht, The Netherlands;4. PamGene International B.V., Wolvenhoek 10, 5211 HH ‘s-Hertogenbosch, The Netherlands |
| Abstract: | A functional detection assay was developed for Escherichia coli secreted shiga-like toxin based on antibody capture and visualization with a multivalent galabiose ligand. It was possible to detect verotoxin in medically relevant E. coli samples in a dose dependent fashion. This method is a new step towards measuring functional protein levels in complex mixtures, which can be used for diagnostic purposes in a clinical setting. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |