Dysfunction of the Scn8a Voltage-gated Sodium Channel Alters Sleep Architecture,Reduces Diurnal Corticosterone Levels,and Enhances Spatial Memory |
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Authors: | Ligia A. Papale Ketema N. Paul Nikki T. Sawyer Joseph R. Manns Sergio Tufik Andrew Escayg |
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Affiliation: | From the ‡Department of Psychobiology, Universidade Federal de São Paulo, São Paulo 04024-002, Brazil.;the Departments of §Human Genetics and ;‖Psychology, Emory University, Atlanta, Georgia 30322, and ;the ¶Department of Anatomy and Neurobiology, Morehouse School of Medicine, Atlanta, Georgia 30310 |
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Abstract: | Voltage-gated sodium channels (VGSCs) are responsible for the initiation and propagation of transient depolarizing currents and play a critical role in the electrical signaling between neurons. A null mutation in the VGSC gene SCN8A, which encodes the transmembrane protein Nav1.6, was identified previously in a human family. Heterozygous mutation carriers displayed a range of phenotypes, including ataxia, cognitive deficits, and emotional instability. A possible role for SCN8A was also proposed in studies examining the genetic basis of attempted suicide and bipolar disorder. In addition, mice with a Scn8a loss-of-function mutation (Scn8amed-Tg/+) show altered anxiety and depression-like phenotypes. Because psychiatric abnormalities are often associated with altered sleep and hormonal patterns, we evaluated heterozygous Scn8amed-jo/+ mutants for alterations in sleep-wake architecture, diurnal corticosterone levels, and behavior. Compared with their wild-type littermates, Scn8amed-jo/+ mutants experience more non-rapid eye movement (non-REM) sleep, a chronic impairment of REM sleep generation and quantity, and a lowered and flattened diurnal rhythm of corticosterone levels. No robust differences were observed between mutants and wild-type littermates in locomotor activity or in behavioral paradigms that evaluate anxiety or depression-like phenotypes; however, Scn8amed-jo/+ mutants did show enhanced spatial memory. This study extends the spectrum of phenotypes associated with mutations in Scn8a and suggests a novel role for altered sodium channel function in human sleep disorders. |
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Keywords: | Ion Channels Mouse Genetics Neurobiology Neurological Diseases Sodium Channels HPA Axis SCN8A Sleep Behavior |
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