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<Emphasis Type="SmallCaps">d</Emphasis>-Psicose production from <Emphasis Type="SmallCaps">d</Emphasis>-fructose using an isolated strain, <Emphasis Type="Italic">Sinorhizobium</Emphasis> sp.
Authors:Deok-Kun Oh  Nam-Hee Kim  Hye-Jung Kim  Chang-Su Park  Seon Won Kim  Minsu Ko  Bueng Wan Park  Min Ho Jung  Ki-Hong Yoon
Institution:(1) Department of Bioscience and Biotechnology, Konkuk University, Seoul , 143-701, Korea;(2) Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Korea;(3) Institute of Molecular Genetics, Solgent Co., Ltd., Daejeon, 305-348, Korea;(4) School of Food Science and Biotechnology, Woosong University, 17-2 Jayang-dong, Dong-gu, Daejeon, 300-718, Korea
Abstract:Sinorhizobium sp., which can convert d-fructose into d-psicose, was isolated from soil. The optimal pH, temperature, and cell concentration for d-psicose production with the isolated strain were 8.5, 40°C, and 60 mg/ml, respectively. The toluene-treated cells showed 2.5- and 4.8-fold increases in the d-psicose concentration and productivity compared with untreated washed cells. Under the optimal conditions, the toluene-treated cells produced 37 g d-psicose/l from 70% (w/v) (3.9 M) d-fructose after 15 h.
Keywords:Biotransformation            d-Fructose" target="_blank">d-Fructose  Isolated strain            d-Psicose" target="_blank">d-Psicose            Sinorhizobium sp  
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