Effect of the fungicide Prochloraz-Mn on the cell wall structure of <Emphasis Type="Italic">Verticillium fungicola</Emphasis> |
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Authors: | D Bernardo M Novaes-Ledieu A Perez Cabo F Gea Alegría C García Mendoza |
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Institution: | 1.Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain,Spain;2.Centro de Investigación, Experimentación y Servicios del Champi?ón, 16220 Quintanar del Rey, Cuenca, Spain,Spain |
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Abstract: | The chemical structure of the cell wall of two isolates of Verticillium fungicola collected from diseased fruit bodies of the commercial mushroom Agaricus bisporus treated with the fungicide Prochloraz-Mn was analyzed. The isolates were obtained during different periods of time and grown
in the absence and presence of the LD50 values of the fungicide for V. fungicola. In addition, another V. fungicola isolate collected previous to the routine utilization of Prochloraz-Mn but grown under the same conditions was also analyzed.
The overall chemical composition of the cell wall from the three isolates showed detectable differences in their basic components,
with a significant decrease in the protein content in fungicide-treated cells. This inhibitory effect was partially compensated
by an increase in neutral and/or aminated carbohydrates and was accompanied by appreciable modifications of polysaccharide
structure, as deduced after methylation analysis and gas-liquid chromatography-mass spectrometry (GLC-MS). Moreover, differences
in hyphal morphology caused by the fungicide were observed by transmission electron microscopy (TEM).
Accepted 2 May 2002
Electronic Publication |
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Keywords: | Verticillium fungicola Cell wall Partial protein inhibition Carbohydrate rearrangement Prochloraz-Mn |
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